It does matter mike, yes, as which way round they go affects the direction of rotation.
The easiest way, I find, to ensure rotation (and polarity) are as required is to hook D and one F lead together, and motor it with a battery using your chosen earth.
If it turns the way it is driven on the bike - clockwise it should be atop an MO1 on a B - then all's well and good. And the polarity will be confirmed.
You can do the same by driving the dyn with a drill or lathe and seeing with a meter which way it generates, and what polarity, but I find a battery quicker and easier.
If it turns the 'wrong way', either swap the F leads round, or swap the brush connections over, between D and earth. Whichever offers the more comfortable life for the various wires. Not both though - or you're back where you started.
Never a bad idea to mark one of the F wires for the next time with a bit of something, and to scribe the brush-holder insulating plate with a D or an E to remind you which side is which with the F wires as you have now got them.